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affigel 15  (Bio-Rad)


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    Bio-Rad affigel 15
    Affigel 15, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affigel 15/product/Bio-Rad
    Average 95 stars, based on 855 article reviews
    affigel 15 - by Bioz Stars, 2026-02
    95/100 stars

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    ( A ) The two mechanistically different routes to resolve UV photolesions: Translesion synthesis (TLS) and Nucleotide Excision Repair (NER). ( B, C , E, and F ) Cellular viability after exposure to increasing UV doses was determined for the indicated cell lines by clonogenic survival assays. Error bars represent one standard error of the mean from at least three independent experiments. In addition t -tests have been carried out for indicated survival curves. P≥0.05 not significant (NS), P≤0.05 statistically significant (*). ( D ) Immunoblot showing PCNA monoubiquitylation in whole cell lysates of wild type and <t>ube2t−/−</t> cells after exposure to UV (60 Jm −2 ) and the indicated recovery time. Immunoblot for β-actin confirms equal protein loading.
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    ( A ) The two mechanistically different routes to resolve UV photolesions: Translesion synthesis (TLS) and Nucleotide Excision Repair (NER). ( B, C , E, and F ) Cellular viability after exposure to increasing UV doses was determined for the indicated cell lines by clonogenic survival assays. Error bars represent one standard error of the mean from at least three independent experiments. In addition t -tests have been carried out for indicated survival curves. P≥0.05 not significant (NS), P≤0.05 statistically significant (*). ( D ) Immunoblot showing PCNA monoubiquitylation in whole cell lysates of wild type and <t>ube2t−/−</t> cells after exposure to UV (60 Jm −2 ) and the indicated recovery time. Immunoblot for β-actin confirms equal protein loading.
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    ( A ) The two mechanistically different routes to resolve UV photolesions: Translesion synthesis (TLS) and Nucleotide Excision Repair (NER). ( B, C , E, and F ) Cellular viability after exposure to increasing UV doses was determined for the indicated cell lines by clonogenic survival assays. Error bars represent one standard error of the mean from at least three independent experiments. In addition t -tests have been carried out for indicated survival curves. P≥0.05 not significant (NS), P≤0.05 statistically significant (*). ( D ) Immunoblot showing PCNA monoubiquitylation in whole cell lysates of wild type and ube2t−/− cells after exposure to UV (60 Jm −2 ) and the indicated recovery time. Immunoblot for β-actin confirms equal protein loading.

    Journal: PLoS ONE

    Article Title: The Fanconi Anaemia Components UBE2T and FANCM Are Functionally Linked to Nucleotide Excision Repair

    doi: 10.1371/journal.pone.0036970

    Figure Lengend Snippet: ( A ) The two mechanistically different routes to resolve UV photolesions: Translesion synthesis (TLS) and Nucleotide Excision Repair (NER). ( B, C , E, and F ) Cellular viability after exposure to increasing UV doses was determined for the indicated cell lines by clonogenic survival assays. Error bars represent one standard error of the mean from at least three independent experiments. In addition t -tests have been carried out for indicated survival curves. P≥0.05 not significant (NS), P≤0.05 statistically significant (*). ( D ) Immunoblot showing PCNA monoubiquitylation in whole cell lysates of wild type and ube2t−/− cells after exposure to UV (60 Jm −2 ) and the indicated recovery time. Immunoblot for β-actin confirms equal protein loading.

    Article Snippet: Antisera were pre-cleared on a GST-coupled Affigel-15 matrix (Biorad) and then affinity purified on an Affigel-15 matrix coupled to UBE2T.

    Techniques: Translesion Synthesis, Western Blot